Optimization and Modelling of The Analysis of Pleuromutilin Antibiotic Extracted From Biomass
O. Benkortbi
Laboratory of Biomaterials and Phenomena of Transport (LBMPT) Dr Yahia Farès de Médéa University, Street Ain of heb, 26000 Médéa, Algeria
M. Hamadache
Laboratory of Biomaterials and Phenomena of Transport (LBMPT), Dr Yahia Fares University, Ain of hab, 26001 Médéa, Algeria
S. Hanini
Laboratory of Biomaterials and Phenomena of Transport (LBMPT), Dr Yahia Fares University, Ain of hab, 26001 Médéa, Algeria
F. Bentahar
Laboratory of Phenomenon of Transfer (LPT), Bab's University to ezzouar, USTHB, BP 32 El Alia, 16110 Alger, Algeria.
A. Amrane
Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, Avenue du Général Leclerc, CS 50837, 35708 Rennes Cedex 7, France. ;European University of Brittany, 5 Bd Laënnec, 35 000 Rennes, France
Corresponding Author(s) : O. Benkortbi
benkortbi_oth@yahoo.fr
Asian Journal of Chemistry,
Vol. 22 No. 8 (2010): Vol 22 Issue 8
An isocratic and simple high performance liquid chromatographic method was developed for the direct resolution of pleuromutilin antibiotic extracted from biomass. The method uses Beckman coulter, ODS, C8, 450 × 4,6 mm I.D. Several mobile phases, flow rates and temperatures were checked in order to optimize the resolution. UV detection at 212 nm was considered. The method proved to be able to resolve pleuromutilin antibiotic. The best base line separation was carried out using mobile phase consisting of acetonitrile and buffer in ratio 60/40, column of C8 at 35 °C. The optimized method proved an important improvement of the retention time, which decreased from a value of 10.8 min found in the available literature to only 2.5 min.
Benkortbi, O., Hamadache, M., Hanini, S., Bentahar, F., & Amrane, A. (2010). Optimization and Modelling of The Analysis of Pleuromutilin Antibiotic Extracted From Biomass. Asian Journal of Chemistry, 22(8), 6044–6052. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/11783
