Optimization and Modelling of The Analysis of Pleuromutilin Antibiotic Extracted From Biomass

Abstract

An isocratic and simple high performance liquid chromatographic method was developed for the direct resolution of pleuromutilin antibiotic extracted from biomass. The method uses Beckman coulter, ODS, C₈, 450 × 4,6 mm I.D. Several mobile phases, flow rates and temperatures were checked in order to optimize the resolution. UV detection at 212 nm was considered. The method proved to be able to resolve pleuromutilin antibiotic. The best base line separation was carried out using mobile phase consisting of acetonitrile and buffer in ratio 60/40, column of C8 at 35 °C. The optimized method proved an important improvement of the retention time, which decreased from a value of 10.8 min found in the available literature to only 2.5 min.