HPTLC Quantitation of Sinigrin from Seed Powder of Brassica juncea (L.) Czern and Brassica nigra (L.)

Abstract

A simple and sensitive high performance thin layer chromatographic (HPTLC) method has been developed for the quantitative determination of sinigrin from seed powder of Brassica juncea (L.) Czern and Brassica nigra (L.). The seeds of both the species were extracted using a hydro-alcoholic mixture and the extracts were separated on HPTLC Silica gel 60 F254, using a mobile phase of ethyl acetate:methanol:water (6:2:1). Detection and quantitation were carried out densitometrically at l = 230 nm. The method was validated in terms of linearity, precision and accuracy. Linear response to sinigrin was obtained over the concentration range of 400- 1200 ng/band. The method proposed for quantitative monitoring of sinigrin from seed powder of Brassica juncea (L.) Czern and Brassica nigra (L.) is simple and accurate and can be used for routine quality testing.