Simultaneous Determination of Irinotecan Hydrochloride and its Related Compounds by High Performance Liquid Chromatography Using Ultraviolet Detection
Ali Mohammadi
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran-14174, Iran ; Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Farnaz Esmaeili
Nanotechnology Research Centre, Tehran University of Medical Sciences, Tehran, PO Box 14155-6451, Iran.
Rassoul Dinarvand
Nanotechnology Research Centre, Tehran University of Medical Sciences, Tehran, PO Box 14155-6451, Iran.
Fatemeh Atyabi
Department of Pharmaceutics, Faculty of Pharmacy, Novel Drug Delivery Systems Laboratory, Tehran University of Medical Sciences, Tehran, P.O. Box: 14155-6451, Tehran-14174, Iran.
Roderick B. Walker
Department of Pharmaceutics, Faculty of Pharmacy, Rhodes University, Grahamstown 6140, South Africa
Corresponding Author(s) : Ali Mohammadi
alimohammadi@tums.ac.ir
Asian Journal of Chemistry,
Vol. 22 No. 5 (2010): Vol 22 Issue 5
A new simple, precise and accurate high performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of irinotecan (CPT-11) and two related compounds viz., 7-ethyl-10-hydroxycamptothecin (SN-38) and camptothecin (CPT) in pharmaceutical dosage forms. Chromatography was accomplished using a reversed-phase C18 column and ultraviolet (UV) detection and an isocratic mobile phase consisting of 3 % v/v triethylammonium acetate buffer (pH 3) and acetonitrile (70:30 v/v). The linear range of quantitation for all the compounds was 0.1-10 μg/mL. The limit of quantitation for all the compounds ranged between 0.01-0.05 μg/mL. The method has the requisite accuracy, selectivity, sensitivity and precision to assay of CPT-11 and related compounds in pharmaceutical dosage forms and bulk API.
Mohammadi, A., Esmaeili, F., Dinarvand, R., Atyabi, F., & B. Walker, R. (2010). Simultaneous Determination of Irinotecan Hydrochloride and its Related Compounds by High Performance Liquid Chromatography Using Ultraviolet Detection. Asian Journal of Chemistry, 22(5), 3966–3972. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/11636
