A New Caffeine Test for Diagnosis of Cirrhosis by GC/MS
C. Mesaros
Department of Biophysics and Medical Biotechnics, University of Medicine and Pharmacy, Targu Mures, Romania.
M. Culea
Department of Biomedical Physics, Babes-Bolyai University, Cluj-Napoca-400084, Romania
A.M. Iordache
Department of Biomedical Physics, Babes-Bolyai University, Cluj-Napoca-400084, Romania ; National R&D Institute of Cryogenics and Isotopic Technologies - ICSI Rm. Valcea, 4 Uzinei St., Rm. Valcea, 240050, Romania
I. Visovan
Department of Biomedical Physics, Babes-Bolyai University, Cluj-Napoca-400084, Romania
C. Cozar
Department of Biomedical Physics, Babes-Bolyai University, Cluj-Napoca-400084, Romania
C. Cosma
Department of Biomedical Physics, Babes-Bolyai University, Cluj-Napoca-400084, Romania
Corresponding Author(s) : M. Culea
mculea@phys.ubbcluj.ro
Asian Journal of Chemistry,
Vol. 22 No. 5 (2010): Vol 22 Issue 5
A method for caffeine levels determination in children suffering from liver diseases by gas chromatography-mass spectrometry (GC/MS) is presented. The selected ion monitoring (SIM) mode was used in caffeine test measurements by adding known amounts of the 15N labelled internal standard. The method was validated in the range 0-20 μg/mL caffeine. A single dose of 4 mg/kg p.o. of caffeine was followed by blood concentrations measurements at two points, 1 and 9 h. Caffeine clearance, 0.74 ± 0.49 mL min-1 kg-1, measured in patients with cirrhosis was reduced and half live time, t1/2 = 14.73 ± 12.36 h, was increased as compared with controls of 1.28 ± 0.31 mL min-1 kg-1 and t1/2 = 5.73 ± 1.58 h.
Keywords
Caffeine testGC-MSCirrhosisClearance
Full Article
Generated from XML file
Mesaros, C., Culea, M., Iordache, A., Visovan, I., Cozar, C., & Cosma, C. (2010). A New Caffeine Test for Diagnosis of Cirrhosis by GC/MS. Asian Journal of Chemistry, 22(5), 3608–3614. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/11589
