Isolation and Characterization of Flavonoids Isolated from Leaves of Benalu Jeruk (Scurrula fusca G. Don) as Antioxidant

Helmina Br. Sembiring; Tonel Barus; Lamek Marpaung; Partomuan Simanjuntak

doi:10.14233/ajchem.2017.20604

Issue

Vol. 29 No. 8 (2017): Vol 29 Issue 8

Issue Published : June 12, 2017

This work is licensed under a Creative Commons Attribution 4.0 International License.

Isolation and Characterization of Flavonoids Isolated from Leaves of Benalu Jeruk (Scurrula fusca G. Don) as Antioxidant

https://doi.org/10.14233/ajchem.2017.20604

Helmina Br. Sembiring

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan, Indonesia

Tonel Barus

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan, Indonesia

Lamek Marpaung

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan, Indonesia

Partomuan Simanjuntak

Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Cibinong, Indonesia; Faculty of Pharmacy, Pancasila University, Jagakarsa, Jakarta Selatan, Indonesia

Corresponding Author(s) : Helmina Br. Sembiring

helmina02@yahoo.com

Asian Journal of Chemistry, Vol. 29 No. 8 (2017): Vol 29 Issue 8
Article Published : June 12, 2017

Abstract

Two flavonoids were isolated from leaves of Benalu Jeruk (Scurrula fusca G. Don) by extracting the leaves powder with methanol. The methanol extract was partitioned with ethyl acetate. The ethyl acetate extract was then partitioned further with mixture of methanol and n-hexane. The methanolic layer was separated and hydrolyzed with 2 N HCl to give flavonoid aglycon. This flavonoid aglycon was purified by column chromatography (SiO₂, chloroform-methanol = 100:0 ~ 80:20) to produce two flavonoids, flavonoid A (3,7-dihydroxy-5-methyl-3’,4’-dihydrofurano-flavonol) and flavonoid B (3,5,7,4’-tetrahydroxyflavonol or kaempferol). The antioxidant activity test for flavonoids A and B gave IC₅₀ 9.383 and 8.713 mg/mL respectively with ascorbic acid as comparison (IC₅₀ 12.08 mg/mL). Chemical structure was determined based on spectroscopy data interpretation [FT-IR, UV, 1-D NMR (¹H NMR, ¹³C NMR and DEPT), 2-D NMR (¹H-¹H COSY, ¹H-¹³C HMQC, ¹H-¹³C HMBC) and LC-MS].

Keywords

Scurrula fusca G. Don 3,7-Dihydroxy-5-methyl-3’,4’-dihydrofurano-flavonol 3,5,7,4’-Tetrahydroxyflavonol Antioxidant

Sembiring, H. B., Barus, T., Marpaung, L., & Simanjuntak, P. (2017). Isolation and Characterization of Flavonoids Isolated from Leaves of Benalu Jeruk (Scurrula fusca G. Don) as Antioxidant. Asian Journal of Chemistry, 29(8), 1743–1745. https://doi.org/10.14233/ajchem.2017.20604

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References

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References

S. Chatterjee, Z. Niaz, S. Gautam, S. Adhikari, P.S. Variyar and A. Sharma, Food Chem., 101, 515 (2007); https://doi.org/10.1016/j.foodchem.2006.02.008.

P. Molyneux, Songklanakarin J. Sci. Technol., 26, 211 (2004).

T. Ishizu, H. Winarno, E. Tsujino, T. Morita and H. Shibuya, Chem. Pharm. Bull. (Tokyo), 50, 489 (2002); https://doi.org/10.1248/cpb.50.489.

H. Shibuya, K. Ohashi and I. Kitagawa, Pure Appl. Chem., 71, 1109 (1999); https://doi.org/10.1351/pac199971061109.

J.P. Fisher, G.K. Phoenix, S.W. Smith, M.C. Press and D.D. Cameron, Redistriguting the Wealth: Interactions Between Plant Parasitism and Parasite Litter in Semi-Natural Grassland Crassland Communities, 11th World Congress on Parasitic Plants, Martina Franca, Italy, 7-12 June (2011).

M.C. Press and G.K. Phoenix, New Phytol., 166, 737 (2005); https://doi.org/10.1111/j.1469-8137.2005.01358.x.

V. Barao, G. Von Arx and G.C.T. Ceccantini, Modification on Wood Hydraulic Conductivity and Embolism Increase in Tipuana Tipu Parasitized by Struthanthus vulgaris, 11th World Congress on Parasitic Plants, Martina Franca, Italy, 7-12 June (2011).

J.B. Runyon, J.F. Tooker, M.C. Mescher and C.M.D. Modreas, Parasitic Plants in Agriculture: Chemical Ecology of Germination and Host- Plant Location as Targets for Sustainable Control: A Review Springer Science+Business Media B.V., pp. 123-136 (2009).

P.J. Deshmukh, Int. J. Adv. Biotechnol. Res., 2, 431 (2011).

F.H. Bamane, J.H. Badr and O.A.R.M. Amin, Afr. J. Biotechnol., 11, 14380 (2012); https://doi.org/10.5897/AJB12.2093.

H.B. Sembiring, T. Barus, L. Marpaung and P. Simanjuntak, Int. J. PharmTech. Res., 8, 24 (2015).

B. Ginting, L. Marpaung, T. Barus and P. Simanjuntak, Asian J. Chem., 28, 199 (2016); https://doi.org/10.14233/ajchem.2016.19342.

J. Dai and R.J. Mumper, Molecules, 15, 7313 (2010); https://doi.org/10.3390/molecules15107313.

K.R. Markham and M.B. Thomas, The Systematic Identification of Flavonoids, Springer-Verlag, New York-Heidelberg-Berlin (1970).

K. Biemann, Spectral Data for Structure Determination of Organic Compounds, Springer-Verlag Berlind Heildelberg (1983).

P.F. Pinheiro and G.C. Justino, in ed. V. Rao, Structural Analysis of Flavonoids and Related Compounds – A Review of Spectroscopic Applications, In: Phytochemicals – A Global Perspective Their Role in Nutrition and Health, InTech, Chap. 2, pp. 33-56 ( 2012).

J.H. Simpson, Organic Structure Determination Using 2-D NMR Spectroscopy A Problem-Based Approach, Elsevier Inc., Canada (2008).

S.P. Piaru, R. Mahmud, A.M.S. Abdul Majid and Z.D. Mahmoud Nassar, Asian Pac. J. Trop. Med., 5, 294 (2012); https://doi.org/10.1016/S1995-7645(12)60042-X.

Issue

Vol. 29 No. 8 (2017): Vol 29 Issue 8

Isolation and Characterization of Flavonoids Isolated from Leaves of Benalu Jeruk (Scurrula fusca G. Don) as Antioxidant

Corresponding Author(s) : Helmina Br. Sembiring

Abstract

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