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Vol. 29 No. 8 (2017): Vol 29 Issue 8

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Determination of Total Phenolics, Total Flavonoids and Evaluation of DPPH Free Radical Scavenging Activity of Ashwagandha (Withania somnifera L.) Roots

https://doi.org/10.14233/ajchem.2017.20537
Susheel Gulati
Medicinal, Aromatic and Potential Crops Section, Old IATTE Building, CCS Haryana Agricultural University, Hisar-125 004, India
V.K. Madan
Medicinal, Aromatic and Potential Crops Section, Old IATTE Building, CCS Haryana Agricultural University, Hisar-125 004, India
Satya Shree Jangra
Medicinal, Aromatic and Potential Crops Section, Old IATTE Building, CCS Haryana Agricultural University, Hisar-125 004, India
I.S. Yadav
Medicinal, Aromatic and Potential Crops Section, Old IATTE Building, CCS Haryana Agricultural University, Hisar-125 004, India

Corresponding Author(s) : V.K. Madan

vikku60@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 8 (2017): Vol 29 Issue 8

Abstract

The present study was performed to estimate the total phenolics, total flavonoids and to evaluate the antioxidant activity of aqueous and ethanolic extracts of ashwagandha (Withania somnifera L.) roots of promising genotypes viz. HWS-08-14, HWS-08-18, HWS-1228, HWS-1229 and Selection-2B & varieties JA-20 and RVA-100. The results revealed that the extract yield of aqueous extracts (9.95 g/100 g) was higher than ethanolic extracts (3.96 g/100 g). Aqueous extracts contained the higher amount of total phenolics (3.58 mg GAE/g d.w.b.) than ethanolic extracts (2.19 mg GAE/g d.w.b.) whereas ethanolic extracts contained the higher amount of total flavonoids (1.16 mg CE/g d.w.b.) than aqueous extracts (0.82 mg CE/g d.w.b.). DPPH free radical scavenging activity of ashwagandha extracts varied widely and it increased with increase of concentration levels. Among aqueous and ethanolic extracts, aqueous extracts exhibited higher antioxidant activity. Hence, aqueous extracts of ashwagandha roots are better source of antioxidants in comparison to ethanolic extracts.

Keywords

Withania somnifera Total phenolics Total flavonoids Antioxidant activity
Gulati, S., Madan, V., Jangra, S. S., & Yadav, I. (2017). Determination of Total Phenolics, Total Flavonoids and Evaluation of DPPH Free Radical Scavenging Activity of Ashwagandha (Withania somnifera L.) Roots. Asian Journal of Chemistry, 29(8), 1660–1664. https://doi.org/10.14233/ajchem.2017.20537
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