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Chemical and Phytochemical Composition of Ashwagandha (Withania somnifera L.) Roots
Corresponding Author(s) : V.K. Madan
Asian Journal of Chemistry,
Vol. 29 No. 8 (2017): Vol 29 Issue 8
Abstract
The present study was undertaken for estimation of chemical and phytochemical composition of ashwagandha (Withania somnifera L.) roots of promising genotypes viz. HWS-08-14, HWS-08-18, HWS-1228, HWS-1229 and selection-2B & varieties JA-20 and RVA-100. Chemical parameters viz. crude fibre, minerals (Fe, Cu, Zn, Mn), starch, total sugars, reducing sugars, non-reducing sugars were analyzed in ashwagandha roots. Crude fibre content ranged from 17.4 to 37.3 %, Fe content ranged from 595.83 to 983.33 ppm, Cu content ranged from 8.40 to 13.72 ppm, Zn content ranged from 16.33 to 41.00 ppm and Mn content ranged from 16.80 to 33.46 ppm. Starch, total sugars, reducing sugars and non-reducing sugars contents ranged from 7.61 to 8.22 mg/g, 5.33 to 6.89 mg/g, 0.40 to 0.64 mg/g and 4.87 to 6.33 mg/g, respectively. Phytochemical parameters viz. total alkaloids and tannins were also analyzed. Total alkaloids and tannins content ranged from 0.26 to 0.31 % and 0.66 to 0.84 mg/g, respectively.
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- M. Garg and J. Ravikant, Pharmacogn. Phytochem., 2, 66 (2014).
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References
A.F. Hill, Economic Botony: A Textbook of Useful Plants and Plant Products, McGraw Hill Book Company Inc; New York, edn 2, pp. 205 (1952).
N. Alam, M. Hossain, M.A. Mottalib, S.A. Sulaiman, S.H. Gan and M.I. Khalil, BMC Complement. Altern. Med., 12, 1175 (2012); https://doi.org/10.1186/1472-6882-12-175.
N. Alam, M. Hossain, M.I. Khalil, M. Moniruzzaman, S.A. Sulaiman and S.H. Gan, BMC Complement. Altern. Med., 11, 65 (2011); https://doi.org/10.1186/1472-6882-11-65.
Q. Uddin, L. Samiulla, V.K. Singh and S.S. Jamil, J. Appl. Pharm. Sci., 2, 170 (2012).
P.K. Khanna, A. Kumar, A. Ahuja and M.K. Kaul, Asian J. Plant Sci., 5, 1061 (2006); https://doi.org/10.3923/ajps.2006.1061.1063.
AOAC, Official Methods of Analysis. Association of Official Analytical Chemists, Washington, DC, edn 17 (2000).
A.J. Maynard, Method in Food Analysis, Academic Press, New York, pp. 176 (1970).
R.M. McCready, J. Guggolz, V. Silviera and H.S. Owens, Anal. Chem., 22, 1156 (1950); https://doi.org/10.1021/ac60045a016.
M.L.C. Jackson, Soil Chemical Analysis, Prentice Hall of India Pvt. Ltd., New Delhi (1973).
W.G.D. Ruig, J. Assoc. Off. Anal. Chem., 69, 1009 (1986).
M. DuBois, K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, Anal. Chem., 28, 350 (1956); https://doi.org/10.1021/ac60111a017.
N. Nelson, J. Biol. Chem., 153, 375 (1944).
M. Somogyi, J. Biol. Chem., 195, 19 (1952).
S.N. Mishra, Quick Method for Estimation of Total Alkaloid in Asgandh, In: Biennial Report of All India Coordinated Research Project on Medicinal and Aromatic Plants, NRCM&AP (Anand), pp. 254 (1996).
R.E. Burns, Agron. J., 63, 511 (1971); https://doi.org/10.2134/agronj1971.00021962006300030050x.
A. Rani, N.R. Baranwal and R.K. Nema, Asian J. Biochem. Pharm. Res., 4, 195 (2012).
M. Garg and J. Ravikant, Pharmacogn. Phytochem., 2, 66 (2014).
S.R. Krishnamurthy and P. Sarala, E-J. Chem., 7, 985 (2010); https://doi.org/10.1155/2010/616851.
S. Johri, U. Jamwal, S. Rasool, A. Kumar, V. Verma and G.N. Qazi, Plant Sci., 169, 1014 (2005); https://doi.org/10.1016/j.plantsci.2005.05.015.
M.O. Viji, M.M. Mathew and R. Parvatham, Vistas, 2, 143 (2013).