Interaction Studies of Bis-(p-aminobenzoato)diaquanickel(II) and Bis-(p-aminobenzoato) triaquacadmium(II) Complexes with Cat Genomic DNA

Abstract

Bis-(p-aminobenzoato)diaquanickel(II) [Ni(p-H₂N-C₆H₄COO)₂· 2H₂O] and bis-(p-aminobenzoato)triaquacadmium(II) [Cd(p-H₂NC₆H₄COO) 2·3H₂O] complexes derived from p-aminobenzoate are reported. The genomic DNA interactions of these complexes with cat genomic DNA have been investigated with UV spectroscopy, gel electrophoresis and viscosity measurements. Ni(H₂N-C₆H₄COO)₂·2H₂O and Cd(H₂NC₆H₄COO) 2·3H₂Ocomplexes exhibit interaction activities with DNA. The experimental results indicate that the complexes can bind to DNA via an intercalative mode. The intrinsic binding constants of the complexes with genomic DNA are 5.2 × 10⁵ - 2.49 × 10⁵ M^-1, respectively.