Parameters of Some of Biochemistry on Ischemia-Reperfusion Injury in Newborn Rat Intestine

Abstract

The purpose of the present study was to determine the effects of treatment with thyroxin and dexamethasone separately and together on I/R injury in newborn rat intestine. Newborn rats were divided into five groups: (1) control (C) group, (2) sham group, (3) thyroxin (T₄) group, (4) dexamethasone (DEX) group and (5) thyroxin plus dexamethasone (T₄+DEX) group. Each group consisted of seven pups. Group T₄ received thyroxin 1 μg/g BW/day, group DEX received dexamethasone 5 μg/gBW/day and group T₄+DEX received 1 μg/gBW/day thyroxin and 5 μg/ gBW/day dexamethasone i.p. for 7 days. Group C received only physiological saline (NaCl 0.9 %). Animals were sacrificed at the end of the reperfusion period and ileum samples were obtained. Malondialdehyde (MDA) levels, a product of lipid peroxidation, glutathione (GSH) levels, a key antioxidant were determined in ileum homogenates. In the jejunum, only T₄ caused a significant statistical elevation in GSH compared with control, sham and DEX groups (p < 0.05). There was significant statistical interaction between T₄ and DEX treatment, i.e. the effect of T₄ treatment was greater in both regions. Mucosal damage scores showed statistical significant effect of T4 in the ileum compared with control and T₄+DEX group. The same significant statistical effect was seen with T₄+DEX group compared to the control group in the ileum. Overall, the comparison between the two regions shows a powerful effect of T₄ in both regions. There was statistical significance between the scores of T₄ in the ileum and of DEX and T₄+DEX in the jejunum. A beneficial effect of thyroxin in all samples was observed in this study supporting its protective effects against I/R injury which was attenuated by glucocorticoid administration.