Simultaneous Estimation and Validation of Tramadol and Paracetamol by HPTLC in Pure and Pharmaceutical Dosage Form

Abstract

A simple rapid, sensitive HPTLC method has been developed and validated for simultaneous estimation of tramadol and paracetamol in pharmaceutical dosage form. It was performed on TLC plate pre-coated with silica getl 60F₂₅₄ as a stationary phase using mobile phase comprising of chloroform:methanol:glacial acetic acid (90:20:1) and the detection was carried out of 270 nm showing R_f value 0.48-0.64 for tramadol and 0.68-0.74 for paracetamol. The percentage estimation of labeled claims of tramadol and paracetamol from marketed tablet was found to be 99.9 and 98.95, respectively. The method was validated in terms of accuracy, precision, specificity of ruggedness. Linearity was observed between 50-200 μg/mL for tramadol and paracetamol. The recoveries of drugs by standard addition method were found in the range of 99.86 and 99.88 for both of the drugs. The proposed method is precise, accurate and can be used to estimate the drug contents of marketed formulations.