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Screening of Antituberculosis Drugs by Thin Layer Chromatography
Corresponding Author(s) : A. Durgbanshi
Asian Journal of Chemistry,
Vol. 29 No. 7 (2017): Vol 29 Issue 7
Abstract
Tuberculosis remains a major public health problem in developing countries. This work demonstrated a new, cost effective, fast and reproducible thin layer chromatographic screening method for the determination of rifampicin, isoniazid and pyrazinamide in pharmaceutical dosages. The solvent system used for the determination of these three drugs is (propanol:hexane:ethyl acetate:ammonia) (5:2:1.7:0.2 v/v) and the Rf value of rifampicin, pyrazinamide and isoniazid was 0.68, 0.74 and 0.50, respectively. The intra-day and inter-day repeatability of the developed method for retention factor showed standard deviation (SD) of approximately 0.5-1.0 %.
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- K. Ilango and S. Arunkumar, Rasayan J. Chem., 3, 493 (2010).
- K. Florey, Analytical Profiles of Drug Substances, Academic Press, New York, p 467 (2005).
- S. Somasundaram, A. Ram and L. Sankaranarayanan, J. Tuber. Res., 2, 40 (2014); https://doi.org/10.4236/jtr.2014.21005.
- Indian Pharmacopoeia, The Controller Publication, Government of India, New Delhi, vol. 3, p. 2054 (2010).
- K.D. Tripathi, Essentials of Medical Pharmacology, Japee Brothers Medical Publishers (P) Ltd., New Delhi, vol. 6, p. 740 (2008).
- S. Puthusseri and M. Mathew, World J. Pharm. Res., 3, 523 (2014).
- Y. Zhang, A. Scorpio, H. Nikaido and Z. Sun, J. Bacteriol., 181, 2044 (1999).
- B.R. Shankar, R. Rajesh and K. Ramya, Asian J. Pharm. Anal. Med. Chem., 8, 4 (2016).
- G.P. Chenna, S.K. Shetty, J.B. Pai, B. Gopinath and M. Ahmed, Int. J. Chemtech Res., 3, 737 (2011).
- H. Wang, C. Cai, C. Chu, J. Liu, Y. Kong, M. Zhu and T. Zhang, Asian J. Pharm. Sci., 7, 303 (2012).
- S. Unsalan, M. Sancar, B. Bekce, P.M. Clark, T. Karagoz, F.V. Izzettin and S. Rollas, Chromatographia, 61, 595 (2005); https://doi.org/10.1365/s10337-005-0549-0.
- S.H. Song, S.H. Jun, K.U. Park, Y. Yoon, J.H. Lee, J.Q. Kim and J. Song, Rapid Commun. Mass Spectrosc., 21, 1331 (2007); https://doi.org/10.1002/rcm.2961.
- A. Srivastava, D. Waterhouse, A. Ardrey and S.A. Ward, J. Pharm. Biomed. Anal., 70, 523 (2012); https://doi.org/10.1016/j.jpba.2012.05.028.
References
K. Ilango and S. Arunkumar, Rasayan J. Chem., 3, 493 (2010).
K. Florey, Analytical Profiles of Drug Substances, Academic Press, New York, p 467 (2005).
S. Somasundaram, A. Ram and L. Sankaranarayanan, J. Tuber. Res., 2, 40 (2014); https://doi.org/10.4236/jtr.2014.21005.
Indian Pharmacopoeia, The Controller Publication, Government of India, New Delhi, vol. 3, p. 2054 (2010).
K.D. Tripathi, Essentials of Medical Pharmacology, Japee Brothers Medical Publishers (P) Ltd., New Delhi, vol. 6, p. 740 (2008).
S. Puthusseri and M. Mathew, World J. Pharm. Res., 3, 523 (2014).
Y. Zhang, A. Scorpio, H. Nikaido and Z. Sun, J. Bacteriol., 181, 2044 (1999).
B.R. Shankar, R. Rajesh and K. Ramya, Asian J. Pharm. Anal. Med. Chem., 8, 4 (2016).
G.P. Chenna, S.K. Shetty, J.B. Pai, B. Gopinath and M. Ahmed, Int. J. Chemtech Res., 3, 737 (2011).
H. Wang, C. Cai, C. Chu, J. Liu, Y. Kong, M. Zhu and T. Zhang, Asian J. Pharm. Sci., 7, 303 (2012).
S. Unsalan, M. Sancar, B. Bekce, P.M. Clark, T. Karagoz, F.V. Izzettin and S. Rollas, Chromatographia, 61, 595 (2005); https://doi.org/10.1365/s10337-005-0549-0.
S.H. Song, S.H. Jun, K.U. Park, Y. Yoon, J.H. Lee, J.Q. Kim and J. Song, Rapid Commun. Mass Spectrosc., 21, 1331 (2007); https://doi.org/10.1002/rcm.2961.
A. Srivastava, D. Waterhouse, A. Ardrey and S.A. Ward, J. Pharm. Biomed. Anal., 70, 523 (2012); https://doi.org/10.1016/j.jpba.2012.05.028.