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Vol. 29 No. 7 (2017): Vol 29 Issue 7

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Synchronised Separation of Antihypertensive, Antidiabetic and Antihyperlipidemic Drugs by RP-HPLC

https://doi.org/10.14233/ajchem.2017.20582
Monika
Department of Pharmaceutical Chemistry, School of Pharmacy, Babu Banarasi Das University, Faizabad Road, Lucknow-226 028, India
Pooja Chawla
Department of Pharmaceutical Chemistry, Roorkee College of Pharmacy, Roorkee-247 667, India
Shailendra Pandey
Department of Pharmacy, Sarojini Naidu Medical College, Agra-282 002, India

Corresponding Author(s) : Monika

Asian Journal of Chemistry, Vol. 29 No. 7 (2017): Vol 29 Issue 7

Abstract

A new simple, rapid and precise reverse phase isocratic HPLC method has been developed for estimation of pioglitazone, amlodipine, atorvastatin and clopidogrel simultaneously. The separation was achieved on a Millenium M-Sil C18 (125 mm × 4.6 mm) column using a mobile phase comprising of methanol:0.02 M KH2PO4 (65:35) and pH adjusted 4.5 with 0.01 % orthophosphoric acid. Stocks and dilutions were prepared in mobile phase. The flow rate was kept at 1 mL/min and analytes were screened with a UV detector. The retention time of pioglitazone, amlodipine, atorvastatin and clopidogrel were 3.17, 4.05, 6.09 and 13.73 min, respectively. The method was validated according to ICH guidelines with respect to linearity, accuracy, precision and specificity. The linearity range was found to be 0.625 to10 μg/mL for all drugs. The limit of detection of pioglitazone, amlodipine, atorvastatin and clopidogrel were 0.11, 0.03, 0.048 and 0.14, respectively and limit of quantitation were 0.34, 0.10, 0.14 and 0.43 μg/mL. The method was validated as a final confirmation of method development with respect to its linearity, precision, accuracy, ruggedness and robustness. This validated method can be handy for the evaluation of various commercially available pharmaceutical dosage forms with high precision and reproducibility.

Keywords

Pioglitazone Amlodipine Atorvastatin Clopidogrel Method development Validation Isocratic separation
Monika, ., Chawla, P., & Pandey, S. (2017). Synchronised Separation of Antihypertensive, Antidiabetic and Antihyperlipidemic Drugs by RP-HPLC. Asian Journal of Chemistry, 29(7), 1573–1577. https://doi.org/10.14233/ajchem.2017.20582
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References
  1. B.L. Kolte, B.B. Raut, A.A. Deo, M.A. Bagool and D.B. Shinde, J. Chromatogr. Sci., 42, 70 (2004); https://doi.org/10.1093/chromsci/42.2.70.
  2. A. Karthik, G. Subramanian, M.C. Rao, K. Bhat, R.A. Kumar, P. Musmade, M. Surulivelrajan, K. Karthikeyan and N. Udupan, Pak. J. Pharm. Sci., 21, 421 (2008).
  3. P. Sripalakit, P. Neamhom and A. Saraphanchotiwitthaya, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 843, 164 (2006); https://doi.org/10.1016/j.jchromb.2006.05.032.
  4. D. Singh, S.C. Dwivedi and A. Kushnoor, Int. J. Biol. Adv. Res., 2, 171 (2010).
  5. P. Shakya and K. Singh, Int. J. Pharm. Sci. Res., 11, 153 (2010).
  6. Z.H. Lin, W. Ji, D. Desai-Krieger and L. Shum, J. Pharm. Biomed. Anal., 33, 101 (2003); https://doi.org/10.1016/S0731-7085(03)00344-3.
  7. S.-Y. Dai, S.-T. Qiu, W. Wu and C.-M. Fu, J. Pharm. Anal., 3, 440 (2013); https://doi.org/10.1016/j.jpha.2013.09.002.
  8. Q. Yu, Z.-Y. Hu, F.-Y. Zhu, J.-H. Zhu, L.-L. Wan, Y. Li and C. Guo, J. Chromatogr. A, 73, 257 (2011); https://doi.org/10.1007/s10337-010-1883-4.
  9. B.G.Chaudhri, N.M. Patel and P.B. Shah, Indian Drugs, 43, 645 (2006).
  10. P. Mishra, A. Gupta and K. Shah, Indian J. Pharm. Sci., 69, 831 (2007); https://doi.org/10.4103/0250-474X.39446.
  11. R. Dhiman, V. Durga and K.M. Meetei, J. Bioanal. Biomed., 7, 91 (2015).
  12. D. Jain, N. Jain and R. Raghuwanshi, Indian J. Pharm. Sci., 70, 263 (2008); https://doi.org/10.4103/0250-474X.41473.
  13. H.M. Hafez, A.E. Abdullah, L.M. Abdelaziz and M.M. Kamal, J. Chromatogr. Sep. Technol., 5, 1 (2014).
  14. H.M. Hafez, A.A. Elshanawany, L.M. Abdelaziz and M.S. Mohram, Pharm. Anal. Acta, 5, 1 (2014).
  15. Y. Shah, Z. Iqbal, L. Ahmad, A. Khan, M.I. Khan, S. Nazir and F. Nasir, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 879, 557 (2011); https://doi.org/10.1016/j.jchromb.2011.01.004.
  16. D. Nagavalli, B. Srinivas and K.C. Chakravarthi, J. Pharm. Biomed. Sci., 4, 1 (2010).
  17. Y. Zhou, J. Li, X. He, M. Jia, M. Liu, H. Li, Z. Xiong, Y. Fan and W. Li, J. Pharm. Biomed. Anal., 83, 101 (2013); https://doi.org/10.1016/j.jpba.2013.04.021.
  18. A. Robinson, J. Hillis, C. Neal and A.C. Leary, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 848, 344 (2007); https://doi.org/10.1016/j.jchromb.2006.10.076.
  19. L. Silvestro, M.C. Gheorghe, I. Tarcomnicu, S. Savu, S.R. Savu, A. Iordachescu and C. Dulea, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 878, 3134 (2010); https://doi.org/10.1016/j.jchromb.2010.09.022.
  20. H. Ksycinska, P. Rudzki and M. Bukowska-Kiliszek, J. Pharm. Biomed. Anal., 41, 533 (2006); https://doi.org/10.1016/j.jpba.2005.11.035.
  21. M. Takahashi, H. Pang, K. Kawabata, N.A. Farid and A. Kurihara, J. Pharm. Biomed. Anal., 48, 1219 (2008); https://doi.org/10.1016/j.jpba.2008.08.020.
  22. ICH Guidelines: Validation of Analytical Procedures: Q2B (1996).
  23. ICH Guidelines: Validation of Analytical Procedures: Q2A (1994).
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