Antioxidant Activity of the Fractions of Cleome gynandra Promotes Antitumor Activity in Ehrlich Ascites Carcinoma

Abstract

The plant Cleome gynandra traditionally used as an Indian medicine to treat microbial diseases. It's anticancer and antioxidant activity is already proved by our research groups; therefore we started to perform column chromatography of methanol extract over silica-gel. Four flavonoid fractions were isolated with mixture of minor compounds and administered at the doses of 150 mg/kg body weight intraperitoneally for 9 consecutive days. Twenty four hours of last dose and 18 h of fasting, the mouse were sacrificed and antitumor effect of all the fractions were assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, biochemical, enzymatic and hematological antioxidant parameters of EAC bearing host. The high amount of antioxidant flavonoid is responsible for significant antitumor activity which correlates its in vitro cytotoxic effect. But the antitumor and cytotoxic activity shows synergistic action along with saponin