Determination of Chlorophyll-a, Pheophytin-a and &beta;-Carotene Contents of Isolated Photosynthetic Reaction Centre Complexes

E.D. Dikio; D.A. Isabirye

Issue

Vol. 23 No. 11 (2011): Vol 23 Issue 11

Issue Published : July 27, 2011

Determination of Chlorophyll-a, Pheophytin-a and β-Carotene Contents of Isolated Photosynthetic Reaction Centre Complexes

E.D. Dikio

Department of Chemistry, Vaal University of Technology, Private Bag X021, Vanderbijlpark 1900, South Africa

D.A. Isabirye

Department of Chemistry, North West University, P.O. Box X2046, Mafikeng 2735, South Africa

Corresponding Author(s) : E.D. Dikio

ezekield@vut.ac.za

Asian Journal of Chemistry, Vol. 23 No. 11 (2011): Vol 23 Issue 11
Article Published : September 7, 2011

Abstract

Stoichiometric quantities of chlorophyll-a, pheophytin-a and β-carotene extracted from spinach leaves have been determined by analysis of the UV-VIS spectrum of extracted pigments. Two methods of using extinction coefficients of purified pigments at different wavelengths are investigated in this paper. The results indicate that these methods are suitable for the routine determination of pigment stoichiometry.

Keywords

Chlorophyll Pheophytin Carotene Photosynthesis Pigment

Dikio, E., & Isabirye, D. (2011). Determination of Chlorophyll-a, Pheophytin-a and β-Carotene Contents of Isolated Photosynthetic Reaction Centre Complexes. Asian Journal of Chemistry, 23(11), 5001–5003. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/11029

Issue

Vol. 23 No. 11 (2011): Vol 23 Issue 11

Determination of Chlorophyll-a, Pheophytin-a and β-Carotene Contents of Isolated Photosynthetic Reaction Centre Complexes

Corresponding Author(s) : E.D. Dikio

Abstract

Keywords

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