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Vol. 29 No. 7 (2017): Vol 29 Issue 7

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A Submicellar Liquid Chromatographic Method for Quantitative Determination of Muscle Relaxant Drug Baclofen in Solubilized System

https://doi.org/10.14233/ajchem.2017.20541
Hitesh Malvia
School of Chemical Sciences, Devi Ahilya University, Khandwa Road, Indore-452 001, India
Ashok Kumar
School of Chemical Sciences, Devi Ahilya University, Khandwa Road, Indore-452 001, India
Pratibha Sharma
School of Chemical Sciences, Devi Ahilya University, Khandwa Road, Indore-452 001, India
Ritesh Mishra
API Research & Development Department, Medilux Laboratories Pvt. Ltd., Pithampur-454 775, India

Corresponding Author(s) : Ashok Kumar

drashoksharma2001@yahoo.com

Asian Journal of Chemistry, Vol. 29 No. 7 (2017): Vol 29 Issue 7

Abstract

A high performance liquid chromatography method, using micellar solution above the critical micellar concentration (CMC) has been developed and validated to quantify baclofen (a muscle relaxant drug) in active pharmaceutical ingredients and pharmaceutical formulations. Process optimization was done at various conditions such as different surfactants, surfactant concentration, pH of buffer solution, solvent composition, solvent selection and different HPLC columns. An isocratic HPLC mode, column temperature 35 °C, Intersil ODS-3 V 250 × 4.6, 5 μm column, 0.8 mL/min mobile phase flow rate and UV detection wavelength at 220 nm was used for the purpose. The method was validated as per International Conference on Harmonization (ICH) guidelines viz., specificity, linearity, range, inter and intra-day precision, accuracy (recovery) and robustness. Forced degradation conditions such as basic, acidic, oxidative, thermal degradation and photolytic are applied to prove the stability indicating nature of the proposed method.

Keywords

Critical micelle concentration Micellar liquid chromatography Sodium dodecyl suphate Baclofen
Malvia, H., Kumar, A., Sharma, P., & Mishra, R. (2017). A Submicellar Liquid Chromatographic Method for Quantitative Determination of Muscle Relaxant Drug Baclofen in Solubilized System. Asian Journal of Chemistry, 29(7), 1509–1514. https://doi.org/10.14233/ajchem.2017.20541
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