Determination of Chlorogenic Acid, Ferulic Acid and Flavonoids in Flos Loniceae by High Performance Liquid Chromatography

Abstract

RP-HPLC method has been developed for the determination of chlorogenic acid, ferulic acid and flavonoids in flos loniceae simultaneously. The extracts was separated on Eclipse XDB-C₁₈ (4.6 × 250 mm, 5 μm) column using acetonitrile and 0.1 % phosphoric acid with gradient elution as the mobile phase at a flow 0.8 mL min^-1, the column temperature was 25 ºC and the detection wavelength was 328 nm. The result indicated that the liner correlation coefficient of all subjects was between 0.9992 and 0.9999. The relative deviation is less than 3.02 % and the recovery range varies from 95.00 to 98.94 %. The method is easy, sensitive, quick, reliable, repeatable and suitable for quality test.