Identification of the Antibacterial Chemical Constituents of Amorpha fruticosa L. by A Modified High-Throughput Screening Method
Diao Yun-Peng
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, P.R. China ; College of Pharmacy, Dalian Medical University, Dalian 116044, P.R. China ; Authors contributed equally to this work.
Li Qing-Gao
Department of Surgery, Dalian Children’s Hospital, Dalian 116012, P.R. China ; Authors contributed equally to this work.
Li Kun
College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, P.R. China
Wang Fei
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, P.R. China
Zhang Jian-Kui
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, P.R. China
Xu Liang
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, P.R. China
Huang Shan-Shan
College of Pharmacy, Dalian Medical University, Dalian 116044, P.R. China
Zhang Hou-Li
College of Pharmacy, Dalian Medical University, Dalian 116044, P.R. China
Kang Ting-Guo
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, P.R. China
Corresponding Author(s) : Kang Ting-Guo
kangtg@126.com
Asian Journal of Chemistry,
Vol. 23 No. 10 (2011): Vol 23 Issue 10
An antibacterial high throughput screening (HTS) method is developed for the Amorpha fruticosa L. fruit extracts, which were tested against methicillin-resistant Staphylococcus aureus (MRSA). The chloroform extract was separated into 80 fractions via preparative high performance liquid chromatography (HPLC). The optimal fractions and tephrosin were tested against methicillinresistant Staphylococcus aureus using high throughput screening method and the antibacterial activity of those selected bioactive fractions was further confirmed by MIC method. Tephrosin exhibited more excellent inhibition ratio than optimal fraction for methicillin-resistant Staphylococcus aureus. Amorpha fruticosa L. exhibited the strongest antibacterial activity and thus the optimal fraction was obtained.
Yun-Peng, D., Qing-Gao, L., Kun, L., Fei, W., Jian-Kui, Z., Liang, X., … Ting-Guo, K. (2011). Identification of the Antibacterial Chemical Constituents of Amorpha fruticosa L. by A Modified High-Throughput Screening Method. Asian Journal of Chemistry, 23(10), 4669–4671. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/10944
