Determination of Glycine in Biological Fluids by Isotopic Dilution Mass Spectrometry

Abstract

A sensitive analytical method for determination of amino acids in biological samples was developed by isotopic dilution mass spectrometry. Quantitative determination of glycine from the resultant tracer spectrum requires deconvolution of the enrichment of the isotopomers. Deconvolution of the ion abundance ratios to yield tracer-to-tracee ratio for the isotopomer was done using Brauman's least squares approach. Comparison with other calculation method is presented. The method was applied for glycine determination in transmembranar transport study by human red cell membrane at different incubation temperatures and at different incubation times.