Spectrophotometric Determination of Copper(II) in Biological Samples by Using 2-Acetylfuran Thiosemicarbazone as Chelating Reagent

Abstract

2-Acetylfuran thiosemicarbazone (AFT) has been proposed a selective reagent for the spectrophotometric determination of copper. 2-Acetylfuran thiosemicarbazone reacts with Cu(II) in the pH range 6.0-8.0 to form yellow coloured complex was measured at different intervals of time at 365 nm, to ascertain the stability of complex. The system obeyed Beer's law upto 0.915 μg mL^-1 of copper(II). The molar absorptivity and Sandell's sensitivity of Cu(II)-2-acetylfuran thiosemicarbazone is 3.33 × 10⁴ Lmol^-1 cm^-1 and 2.0 × 10^-3 μg cm^-2 at 365 nm. The method has been applied for the determination of copper in alloys and in biological samples.