Phytochemical Profiles and Antioxidant Properties of Sarcopyramis bodinieri var. delicata

Abstract

Sarcopyramis bodinieri var. delicata was extracted with 60 % aqueous ethanol and the extract was successively isolated by liquid-liquid partition to yield the extract. Total flavonol content and antioxidant properties of the three organic solvent extracts were conducted, the antioxidant activities were evaluated by measuring the ability of the extracts to scavenge the DPPH and ABTS•+ free radical. In addition, the reducing power, phosphomolybdenum reduction and Fe²⁺ chelation were also assessed. Among those organic fractions, the ethyl acetate fraction (EAF) exhibited the highest total flavonol content, which possessed significant free radical scavenging and antioxidant properties. Seven phenolic compounds were identified by LC-DAD-MS and NMR spectrum from ethyl acetate fraction. Additionally, four major aglycon compounds (quercetin, isorhamnetin, kaempferol and ellagic acid) were determined from hydrolyzed ethyl acetate fraction. The results obtained in vitro models clearly suggest that the ethyl acetate fraction from the extract of Sarcopyramis bodinieri var. delicata is a promising natural source for antioxidants.