Spectrophotometric Determination of Mesna in Commercial Injections

Abstract

A new visible spectrophotometric method for the determination of mesna was carried out based on the reaction of ferric solution with mesna to produce ferriferrous ion. The dependence of the absorbance on pH, temperature and reaction time was investigated. In Clark-lubs buffer medium, the red complex with the absorbance decreased obviously at 480 nm. The decrease value of the absorbance (DA), due to the presence of mesna was correlated with its concentration. Linear relationship with good correlation coefficient (0.9991) was found between DA and the concentration of mesna in a concentration range of 1-30 μg mL^-1. The assay limit of determination was 0.31 μg mL^-1. The proposed method was successfully applied to the determination of the investigated drug in injections with the recoveries of the range from 99.4 to 100.2 %.