A simple and rapid liquid chromatographic methods for enantioselective separation and determination of -(±)-enantiomers of pirbuterol in bulk drugs and formulations using UV and polarimetric detectors connected in series was developed. The enantiomers were tested on numerous commercial HPLC columns. Pirbuterol separation was determined on amylose chiralpak AD-H column. The mobile phase compositions were systematically studied to obtain the optimal chromatographic methods. Validation of methods in selected conditions shows that the chosen methods are selective and precise with linear response of detector for both pairs of enantiomers.
Padmanabha Reddy, Y., Mohana Vamsi, N., Shrivas, M., & Sunil Kumar, M. (2011). Enantioselective Separation and Determination of Pirbuterol in Bulk Drugs and Formulations by HPLC. Asian Journal of Chemistry, 23(8), 3509–3512. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/10658
