Spectrophotometric Determination of Centrophenoxine Hydrochloride with Alkalinity Potassium Permanganate Method

Abstract

The simple, senstitive spectrophotometric methods have been developed for the assay of centrophenoxine hydrochloride in pure and pharmaceutical formulations. This method is based on oxidation of centrophenoxine hydrochloride by potassium permanganate in alkalinity media with the maximum wavelength of colour reaction at 430 and 610 nm, of fading reaction at 525 nm. Reaction conditions were optimized to obtain the maximum colour intensity and maximum fading intensity. The absorbance was found to increase linearly in concentration of centrophenoxine hydrochloride, which was corroborated by the calculation coefficient values (0.9997, 0.9993 and 0.9995, respectively). The system obeyed Beer's law in the range of 2.5-15 μg/mL for centrophenoxine hydrochloride. Various analytical parameters have been evaluated and the results have been validated by statistical data.