Partly-Immersed Three Phase Hollow Fiber Liquid Phase Microextraction of Semi-Volatile Organic Compounds Combined with High Performance Liquid Chromatography

Abstract

A novel method for fast determination of nicotine in tobacco and cigarette was established by using high performance liquid chromatography (HPLC) coupled with a partly immersed three phase hollow fiber liquid phase microextraction (PILPME) technique for sample preparation. The model of partly immersed was introduced for the first time. This study has demonstrated that PILPME constituted a real alternative to the other liquid phase microextraction methods. Parameters related to PILPME (organic solvent, pH of donor and acceptor phase, the proportion of hollow fiber in gas and liquid, extraction time, temperature) were optimized experimentally, for preconcentration and extraction of nicotine in tobacco and cigarette samples has shown many advantages over traditional sample preparation technique, which provided a good linear range (0.05-10 μg/mL) with r² = 0.9996, a low detection limit (5 ng/mL, S/N = 3) and a satisfactory relative standard deviation (< 8 %). The result showed 3.03 and 3.39 mg/g in tobacco and 1.51, 1.45 and 1.73 mg/g in cigarette, respectively. The recovery was obtained in the range of 95.91-97.95 %.