Spectrofluorimetric Determination of Pioglitazone Hydrochloride and Glimepiride in Their Formulations and Biological Fluids

Abstract

A convenient and sensitive spectrofluorimetric method is described for the determination of two antidiabetic drugs i.e., pioglitazone HCl and glimepiride, in pharmaceutical formulations and biological fluids. The method is based on the native fluorescence of the studied drugs in methanol. The fluorescence intensity was measured in methanol at 512 and at 522 nm for pioglitazone HCl excitation and glimepiride, respectively. The effect of some surfactants on the fluorescence intensity was studied. Regression analysis showed good correlation (r = 0.9999) between fluorescence intensity and concentration over the range of 0.005-1.3 μg/mL for pioglitazone HCl with lower limit of detection (LOD) of 1.61 × 10^-3 μg/mL and 0.01-1.5 μg/mL with (LOD) 3.59 × 10^-3 μg/mL for glimepiride. The studied drugs were successfully determined in their tablets and in biological fluids.