In vitro Assessment of Relief to Oxidative Injury by Different Samples of Xanthoxylum armatum

Abstract

The methanolic extract of Zanthoxylum armatum DC was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sucessively. The antioxidant potential of the crude methanolic extract, all the organic fractions and the remaining aqueous fraction was evaluated by four methods: DPPH free radical scavenging activity, total antioxidant activity, FRAP assay and ferric thiocyanate assay and total phonolics were also determined. All the samples showed remarkable antioxidant potential. The results revealed that ethyl acetate fraction showed highest value of percentage inhibition of DPPH radical (92.96 % ± 0.13) with very low concentration (60 μg/mL), IC₅₀ of ethyl acetate fraction was found to be 19.76 ± 0.12 μg/mL, relative to butylated hydroxytoluene (BHT), having IC₅₀ of 12.1 ± 0.92 μg/mL. The chloroform fraction exhibited highest total antioxidant activity (0.9101 ± 0.3) as compared to other fractions. The ethyl acetate fraction also displayed highest FRAP value (714.45 ± 2.61 μg of trolox equivalents) and total phenolic contents (416.4 ± 7.73 mg of gallic acid equivalents). The n-hexane fraction showed highest value of inhibition of lipid peroxidation (57.51 % ± 1.56).