Study on Solid-Phase Extraction and Ultra Performance Liquid Chromatography for the Determination of Aristolochic acid A in Herbal Plants

Abstract

A solid-phase extraction and ultra performance liquid chromatography for the determination of aristolochic acid A in herbal plants was studied in this manuscript. The aristolochic acid A was extracted from samples by 33 % methanol (containing 2 % formic acid). The extraction was purified by strong anion-exchange reversed-phase extraction cartridge (Oasis MAX extraction cartridge). Some interferences could be removed from the samples by this procedure. Under optimum condition of ultra performance liquid chromatography-diode array detection (UPLCPDA), a baseline separation was achieved for aristolochic acid A within 7 min. The linearity of aristolochic acid A was in the range of 32.27-1236 ng/mL. The detection limit (S/N = 3) was 1.65 ng/mL for aristolochic acid A. The relative standard derivation of overall intra-day variations were less than 2.0 % and the relative standard derivation of inter-day variations were less than 2.5 %. The average recovery factors were ranged from 91-102 %. This method is simple, rapid and sensitive to detect aristolochic acid A in herbal plants.