Pharmacokinetics of Cefaclor in Stray Dogs Determined Microbiologically

Abstract

Pharmacokinetic of cefaclor were evaluated in healthy stray dogs after oral administration. Serial blood samples were collected in heparinized tubes at pre-scheduled time intervals and analyzed by standard NCCLS microbiological assay against gram negative and gram positive bacterial strains by disc diffusion method. Peaked concentration (12.10 μg/mL) of cefaclor was found to be after 2 h. Average plasma concentration remained above minimum inhibitory concentration (MIC) value from 0.5 to 12.0 h. The values of maximum plasma concentration (C_max) were found to be 5.21, 7, 8.20 and 7.67 mg/L and time of peak (T_max) 2.65, 1.56, 1.85 and 1.73 h against Escherichia coli, Staphalococuss aureus, Pasturella multocida and Basillus subtilis, respectively. Absorption half life (t_1/2) was found to be 1.38, 0.42, 0.67 and 0.43 h against four strains, respectively. Pharmacokinetics was found insignificant (p < 0.05) in case of gender. Results showed that oral administration of 375 mg cefaclor as tablet thrice daily might be maintain substantial concentration that prove it to be effective for the treatment of specific infections in dogs.