Standardization of Marketed Adulsa Syrup Containing Vasaka by High Performance Thin Layer Chromatography

Abstract

Adulsa syrup is a marketed Ayurvedic formulation containing vasaka as one of the main ingredient. Present study aims to standardize this formulation by HPTLC finger printing of vasicine fraction, isolated from authenticated powder of Adhatoda vasica and characterized by IR, UV and HPLC. Three extracts, namely aqueous extract with 5 % citric acid, acetone:alcoholic (1:1) extract and alcoholic extract 90 % of powder have been prepared and evaluated. Acetone:alcohol (1:1) extract indicated presence of maximum amount of vasicine. Densitometric scan showed three well-resolved peaks at 254 nm. The central peak was integrated as vasicine. The other two peaks were ketone which was proved by dinitrophenylhydrazine test. Standard calibration curve was prepared using isolated vasicine fraction as a marker. Calibration plots were linear in the range 4-12 μg/spot with correlation coefficient 0.9982. Recovery of vasicine was 103.82 %. Stress stability study of vasicine was carried out using acid, alkali and photodegradation. The suitability of this HPTLC method for quantitative determination of compound was proved by validation in accordance with the requirements of pharmaceutical regulatory standards.