Estimation of Acipimox in Bulk Drug and Capsules by RP-HPLC Method

Abstract

A reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of acipimox in bulk drug and pharmaceutical dosage forms. The quantification was carried out on Luna C18 column in isocratic mode, with mobile phase consisting of 0.1 % v/v phosphoric acid in water and acetonitrile in the ratio of 95:5 [v/v]. The mobile phase was pumped at a rate of 1.0 mL/min and the detection was carried out at 229 nm and the linearity was found to be in the range of 20-300 μg/mL. The regression equation was found to be Y = 33672x + 6367.2 with correlation coefficient [r²] of 0.9998. The percentage recovery values were found to be in the range of 99.96-100.07 %. Validation of the proposed method has also been done.