Separation and Simultaneous Quantification of a- and b-Asarone in Acorus calamus Linn. From Indian Sub-Continent on Caffeine Modified Silica
M. Singh1
1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi- 110 062, India
2Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110 062, India
*Corresponding author: E-mail: sahmad_jh@yahoo.co.in
Y.T. Kamal1
1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi- 110 062, India
2Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110 062, India
*Corresponding author: E-mail: sahmad_jh@yahoo.co.in
R. Parveen2
1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi- 110 062, India
2Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110 062, India
*Corresponding author: E-mail: sahmad_jh@yahoo.co.in
Sayeed Ahmad1
1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi- 110 062, India
2Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110 062, India
*Corresponding author: E-mail: sahmad_jh@yahoo.co.in
Asian Journal of Chemistry,
Vol. 23 No. 5 (2011): Vol 23 Issue 5
A simple and validated HPTLC method was applied on caffeine modified silica using toluene:ethyl acetate (93:7 v/v) as mobile phase for simultaneous determination of a- and b-asarone, two major isomeric phytoconstituents of Acorus calamus Linn (Family: Araceae). The mobile phase gave well seperated and compact spots for both a- and b-asarones at retardation factor (Rf) of 0.67 ± 0.02 and 0.77 ± 0.02, respectively over modified silica plates. Densitometric analysis of asarones (a- and b) was carried out in the absorbance mode at 313 nm in the concentration range 50-1000 ng/spot and 10-1000 μg/spot for a- and b asarone, respectively.
Singh1, M., Kamal1, Y., Parveen2, R., & Ahmad1, S. (2011). Separation and Simultaneous Quantification of a- and b-Asarone in Acorus calamus Linn. From Indian Sub-Continent on Caffeine Modified Silica. Asian Journal of Chemistry, 23(5), 2046–2048. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/10286
