Separation and Quantification of a Natural Drug Component Against Human Pathogens from Aristolochia bracteolata Lam

Abstract

Aristolochia bracteolata Lam, whole plant was subjected to Soxhlet extraction with solvents like chloroform, acetone and methanol. Subsequently the extracted solvent phases were passed through thin layer chromatography and three distinct spots were separated as C₁, A₁ and M₂, respectively. Among all the three independent compounds C₁, A₁ compounds showed maximum level of antimicrobial activity against the bacterial pathogens like methicillin resistant Staphylococcus aureus (MRSA), Salmonella typhi and Aspergillus mucor. Among all tested compounds C₁ and M₂ showed ten fold increased activity than A₁ compound. The ¹H NMR analysis of the purified active compounds C₁, A₁ and M₂, clearly shows that all the three compounds are independent. The HPLC and NMR results confirm that these three compounds are pure and entirely different from each other. ¹H NMR analysis individually confirmed that C₁, A₁ and M₂ compounds are pure and aliphatic in nature.