Detection of Galanthamine in Bulbus lycoridis radiatae by Capillary Electrophoresis with an End-column Electrochemiluminescence of Tris(2,2'-bipyridyl)ruthenium(II)
Q. Xiang
Department of Applied Chemistry, Changchun Institute of Technology, Changchun, P.R. China
H. Wang
College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P.R. China
Y. Xu
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P.R. China
L. Sun
Department of Applied Chemistry, Changchun Institute of Technology, Changchun, P.R. China
Y. Gao
Department of Applied Chemistry, Changchun Institute of Technology, Changchun, P.R. China ; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P.R. China
Corresponding Author(s) : Y. Gao
gaoy5680@163.com
Asian Journal of Chemistry,
Vol. 23 No. 4 (2011): Vol 23 Issue 4
A capillary electrophoresis with an end-column electrochemiluminescence detection method is described for the determination of galanthamine in Bulbus lycoridis radiatae without derivatization. To minimize the matrix effect and get rid of the interfering substances in crude extract of the herb, the effect of running buffer on separation and detection of galanthamine in real sample was investigated. Under the optimized conditions: electrochemiluminescence detection at 1.2 V, 15 mmol/L phosphate buffer at pH 8.49, 13 kV separation voltage, the calibration of galanthamine was linear (r = 0.998) with detection limit of 1.0 × 10-9 mol/L.
Xiang, Q., Wang, H., Xu, Y., Sun, L., & Gao, Y. (2010). Detection of Galanthamine in Bulbus lycoridis radiatae by Capillary Electrophoresis with an End-column Electrochemiluminescence of Tris(2,2’-bipyridyl)ruthenium(II). Asian Journal of Chemistry, 23(4), 1553–1556. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/10247
