An isocratic chiral stationary phase high-performance liquid chromatographic (CSP-HPLC) method has been developed and validated for the quantitation of (S)-isomer in eletriptan hydrobromide. Separation was achieved with a Chiralpak AD column. The ratio of n-hexane, ethanol, diethyl amine and trifluoroacetic acid in the mobile phase were optimized to obtain the best separation. UV detection was performed at 223 nm. The described method is linear over a range of limit of quantification - 1.5 μg/mL of (S)-isomer. The mean recovery of (S)-isomer was found to be in the range of 101-102 %. The method is simple, rapid, accurate, selective and precise, useful in the quality control of bulk manufacturing.
Phani Kumar, A., Ganesh, V., Hari Prasad, K., Hariharakrishnan, V., Dubey, P., & Venugopala Rao, B. (2010). HPLC Method for the Enantiomeric Purity of Eletriptan Hydrobromide. Asian Journal of Chemistry, 23(4), 1621–1624. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/10246
