Quantitative Determination of Umbelliferone in the Root Extract of Cichorium intybus by HPTLC

Abstract

A simple sensitive HPTLC method developed for determination of umbelliferone in the methanolic root extract of Cichorium intybus by HPTLC. The stationary phase was precoated silica gel 60 F₂₅₄ and the mobile phase used was toluene:ethyl acetate:glacial acetic acid (8:13:1 v/v/v). The plate was scanned and quantified at 356 nm for umbelliferone. The method was validated in terms of linearity, accuracy and specificity. The proposed HPTLC method provides a faster and cost effective control for routine analysis of umbelliferone in the extracts containing Cichorium intybus coumarins.