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Vol. 37 No. 1 (2025): Vol 37 Issue 1, 2025

Copyright (c) 2024 Ekta Sharma, Damandeep Kaur, Moyad Shahwan, Ritu Chauhan, Hardeep Singh Tuli, Abhishek Chauhan, Reena V. Saini

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Cellular Antioxidant Potential and Inhibition of the Enzymatic Activities Mediated through Achyranthes aspera Phytocomponents

https://doi.org/10.14233/ajchem.2025.32795
Ekta Sharma
Department of Bio-Sciences & Technology, MMEC, Maharishi Markandeshwar (Deemed to Be University), Mullana-133207, India
https://orcid.org/0009-0008-8106-9005
Damandeep Kaur
University Center for Research & Development (UCRD), Chandigarh University, Gharuan, Mohali-140413, India; Centre for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai-602105, India
https://orcid.org/0009-0009-3075-2661
Moyad Shahwan
Department of Clinical Sciences, College of Pharmacy and Health Sciences, Ajman University, Ajman 346, United Arab Emirates; Centre of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman 346, United Arab Emirates
https://orcid.org/0000-0001-8367-4841
Ritu Chauhan
Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun-248002, India
https://orcid.org/0000-0002-9056-6619
Hardeep Singh Tuli
Department of Bio-Sciences & Technology, MMEC, Maharishi Markandeshwar (Deemed to Be University), Mullana-133207, India
https://orcid.org/0000-0003-1155-0094
Abhishek Chauhan
Amity Institute of Environmental Toxicology Safety and Management, Amity University, Noida-201301, India
https://orcid.org/0000-0001-6475-1266
Reena V. Saini
Department of Bio-Sciences & Technology, MMEC, Maharishi Markandeshwar (Deemed to Be University), Mullana-133207, India
https://orcid.org/0000-0002-9025-0702

Corresponding Author(s) : Reena V. Saini

reenavohra10@gmail.com

Asian Journal of Chemistry, Vol. 37 No. 1 (2025): Vol 37 Issue 1, 2025

Abstract

Various medicines of plant origin have been widely used, by many individuals, showing effective and safe results in combating diseases. Similarly, Achyranthes aspera is among these plants which have many potentials. Different concentrations of solvents (water and ethanol) were used to prepare the extracts A. aspera seeds. The phytochemical analysis of these extracted was conducted to evaluate the phenolic, flavonoid and condensed tannin contents. However, 25% hydroalcoholic extract of A. aspera (25% HA-Aca) exhibited the highest antioxidant potential by DPPH (IC50 4.17 ± 1.73 mg/mL), ABTS (IC50 117.33 ± 3.02 mg/mL), lipase (IC50 114.4 mg/mL) and α-amylase (IC50 105.4 mg/mL) inhibition activity using in vitro assays. Moreover, a cellular antioxidant assay was also conducted in 3T3-L1 cells revealed that the cells pre-treated with various A. aspera extracts were effective against the oxidative stress. Also, 25% HA-Aca displayed highest free radicals scavenging capability in the cellular antioxidant assay as compared to the other extracts. Stigmasterol, dolichol phosphate and 9'-hydroxy-9'-apo-epsilon-caroten-3-one are the major compounds present in the 25% HA-Aca extract as identified by LC-MS method. These findings suggest that A. aspera phytocomponents can modulate the redox status and regulate the lipid and carbohydrate metabolism, which might help in obesity.

Keywords

Phytochemicals Antioxidant activity Phenolic compounds Lipase α-Amylase LC-MS method
Sharma, E., Kaur, D., Shahwan, M., Chauhan, R., Tuli, H. S., Chauhan, A., & Saini, R. V. (2024). Cellular Antioxidant Potential and Inhibition of the Enzymatic Activities Mediated through Achyranthes aspera Phytocomponents. Asian Journal of Chemistry, 37(1), 215–221. https://doi.org/10.14233/ajchem.2025.32795
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