Copyright (c) 2024 Nyamdemberel Tsagaanbaatar
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Study of Phytochemistry and Antioxidant Activity of Juniperus sabina L. Grown in Mongolia
Corresponding Author(s) : Nyamdemberel Tsagaanbaatar
Asian Journal of Chemistry,
Vol. 37 No. 1 (2025): Vol 37 Issue 1, 2025
Abstract
In traditional Mongolian medicine, J. sabina L. fruits and leaves are used as medicinal raw materials to treat kidney, joint and respiratory diseases. The present study considers the phytochemical investigation of the plant. TLC test results identified gallic acid, quercetin, kaempferol, umbelliferone and 1,8-cineole in J. sabina L. Quantitative determination by UV spectrophotometry showed the content of polyphenolic compounds to be 127-195 mg GAE/g, total flavonoids 46-59.1 mg QE/g and total coumarins 14.8-20.5 mg UE/g. Plant antioxidant activity was assessed using the free radical reduction DPPH test and the 50% inhibitory concentration was determined at a 200 µg/mL concentration, ethyl acetate and methanol fractions of J. sabina L. exhibited free radical scavenging activity of 83.15% ± 1.85 and 46.14% ± 1.08, respectively. The 50% free radical inhibition concentration values were 112.8 ± 1.99 µg/mL and 195.2 ± 1.57 µg/mL, both less than 200 µg/mL, indicating antioxidant activity.
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- T. Narangerel and D. Mankowska, Biotechnol. Food Sci., 83, 31 (2019); https://doi.org/10.34658/bfs.2019.83.1.31-36
- L.S. Esteban, I. Mediavilla, V. Xavier, T.C.S.P. Pires, R.C. Calhelha, C. López and L. Barros, Molecules., 28, 4448 (2023); https://doi.org/10.3390/molecules28114448
- N. Zivic, S. Milosevic, V. Dekic, B. Dekic, N. Ristic, M. Ristic and L. Sretic, Czech J. Food Sci., 37, 351 (2019); https://doi.org/10.17221/28/2019-CJFS
- D. Kavaz and R.E. El Faraj, Sci. Rep., 13, 7193 (2023); https://doi.org/10.1038/s41598-023-34281-x
- H.N. Gök, N. Orhanet, B. Özüpek, S. Pekacar, S.N. Selvi and D.D. Orhan, Iran. J. Pharm. Res., 20, 441 (2021); https://doi.org/10.22037/ijpr.2021.114838.15055
- N. Orhan, D.D. Orhan, A. Gökbulut, M. Aslan and F. Ergun, Iran. J. Pharm. Res., 16, 64 (2017); https://doi.org/10.22037/ijpr.2017.1962
- J. Asili, S.A. Emami, M. Rahimizadeh, B.S. Fazly-Bazzaz and M.K. Hassanzadeh, J. Essent. Oil-Bear. Plants, 13, 25 (2010); https://doi.org/10.1080/0972060X.2010.10643787
- S. Xu, Q. Chen, N. Luo, J. Yang and D. Li, Front. Plant Sci., 14, 1174922 (2023); https://doi.org/10.3389/fpls.2023.1174922
- U. Ligaa, B. Davaasuren and N. Ninjil, Medicinal Plants of Mongolia used in Western and Eastern Medicine, Admon Press: Ulaanbaatar, Mongolia, p. 65 (2005).
- B. Khurelchuluun and U. Batchimeg, Reference of Raw Materials of Some Medicinal Plants of Mongolia, Mongolian University of Science and Technology: Ulaanbaatar, Mongolia, p. 6 (2006).
- World Health Organization Regional Office for Western Pacific, Medicinal Plants in Mongolia, WHO Press: Switzerland, p. 95 (2013).
- Ts. Khaidav and N. Tumurbaatar, Medicinal Things of Tree Seeds used in Medicine of Mongolia and Tibet, Health Sciences University of Mongolia, Ulaanbaatar, p. 40 (1996).
- V.L. Komarov, Flora of U.S.S.R, English translation, vol. 1, pp. 141-142 (1968).
- O. P. Agrawal, Santosh Bharadwaj, R. Mathur, Planta Med., 40, 98 (1980); https://doi.org/ 10.1055/s-2008-1075011
- Anonymous, Physician’s Desk Reference (PDR) for Herbal Medicines, Thomson Healthcare, Montvale, edn. 3, pp. 477–481 (2004).
- J. Lust, The Herb Book, Bantam Books: New York (1982).
- R. Chiej, The Macdonald Encyclopaedia of Medicinal Plants, Edinburgh: MacDonald (1984).
- M.S. Abdel-Kader, M.T. Alanazi, A.S.B. Saeedan, F.I. Al-Saikhan and A.M. Hamad, J. Pharm. Pharmacogn. Res., 5, 29 (2017); https://doi.org/10.56499/jppres16.151_5.1.29
- National Pharmacopoeia of Mongolia, Soyombo Press: Ulaanbaatar, edn. 1, pp. 582-589 (2011).
- Pharmacopoeia of Raw Materials and Drugs used in Traditional Mongolian Medicines, Institute of Traditional Medicine and Technology of Mongolia, Jicom Press: Ulaanbaatar, p. 25-26,38,41,85, 93 (2023).
- N. Tsagaanbaatar, U. Dashzeveg, N. Nyamdemberel, M. Urtnasan and C. Chimedtseren, Biomed. Pharmacol. J., 17, 1279 (2024); https://doi.org/10.13005/bpj/2941
- H. Wagner and S. Bladt, Plant Drug Analysis: A Thin Layer Chromato-graphy Atlas, Springer-Verlag: Berlin, edn 2 (1996).
- M. Ennajar, J. Bouajila, A. Lebrihi, F. Mathieu, M. Abderraba, A. Raies and M. Romdhane, J. Food Sci., 74, M364 (2009); https://doi.org/10.1111/j.1750-3841.2009.01277.x
- E. Selenge, G. Odontuya and J. Batkhuu, Proc. Mong. Acad. Sci., 195, 48 (2010).
- G.V. Radha, B. Sadhana, S.K. Trideva and S. Ganapaty, J. Pharmacogn. Phytochem., 8, 59 (2019).
- J. Sukhdolgor, Plant Chemistry and Biochemistry, Ulaanbaatar (2013).
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References
T. Narangerel and D. Mankowska, Biotechnol. Food Sci., 83, 31 (2019); https://doi.org/10.34658/bfs.2019.83.1.31-36
L.S. Esteban, I. Mediavilla, V. Xavier, T.C.S.P. Pires, R.C. Calhelha, C. López and L. Barros, Molecules., 28, 4448 (2023); https://doi.org/10.3390/molecules28114448
N. Zivic, S. Milosevic, V. Dekic, B. Dekic, N. Ristic, M. Ristic and L. Sretic, Czech J. Food Sci., 37, 351 (2019); https://doi.org/10.17221/28/2019-CJFS
D. Kavaz and R.E. El Faraj, Sci. Rep., 13, 7193 (2023); https://doi.org/10.1038/s41598-023-34281-x
H.N. Gök, N. Orhanet, B. Özüpek, S. Pekacar, S.N. Selvi and D.D. Orhan, Iran. J. Pharm. Res., 20, 441 (2021); https://doi.org/10.22037/ijpr.2021.114838.15055
N. Orhan, D.D. Orhan, A. Gökbulut, M. Aslan and F. Ergun, Iran. J. Pharm. Res., 16, 64 (2017); https://doi.org/10.22037/ijpr.2017.1962
J. Asili, S.A. Emami, M. Rahimizadeh, B.S. Fazly-Bazzaz and M.K. Hassanzadeh, J. Essent. Oil-Bear. Plants, 13, 25 (2010); https://doi.org/10.1080/0972060X.2010.10643787
S. Xu, Q. Chen, N. Luo, J. Yang and D. Li, Front. Plant Sci., 14, 1174922 (2023); https://doi.org/10.3389/fpls.2023.1174922
U. Ligaa, B. Davaasuren and N. Ninjil, Medicinal Plants of Mongolia used in Western and Eastern Medicine, Admon Press: Ulaanbaatar, Mongolia, p. 65 (2005).
B. Khurelchuluun and U. Batchimeg, Reference of Raw Materials of Some Medicinal Plants of Mongolia, Mongolian University of Science and Technology: Ulaanbaatar, Mongolia, p. 6 (2006).
World Health Organization Regional Office for Western Pacific, Medicinal Plants in Mongolia, WHO Press: Switzerland, p. 95 (2013).
Ts. Khaidav and N. Tumurbaatar, Medicinal Things of Tree Seeds used in Medicine of Mongolia and Tibet, Health Sciences University of Mongolia, Ulaanbaatar, p. 40 (1996).
V.L. Komarov, Flora of U.S.S.R, English translation, vol. 1, pp. 141-142 (1968).
O. P. Agrawal, Santosh Bharadwaj, R. Mathur, Planta Med., 40, 98 (1980); https://doi.org/ 10.1055/s-2008-1075011
Anonymous, Physician’s Desk Reference (PDR) for Herbal Medicines, Thomson Healthcare, Montvale, edn. 3, pp. 477–481 (2004).
J. Lust, The Herb Book, Bantam Books: New York (1982).
R. Chiej, The Macdonald Encyclopaedia of Medicinal Plants, Edinburgh: MacDonald (1984).
M.S. Abdel-Kader, M.T. Alanazi, A.S.B. Saeedan, F.I. Al-Saikhan and A.M. Hamad, J. Pharm. Pharmacogn. Res., 5, 29 (2017); https://doi.org/10.56499/jppres16.151_5.1.29
National Pharmacopoeia of Mongolia, Soyombo Press: Ulaanbaatar, edn. 1, pp. 582-589 (2011).
Pharmacopoeia of Raw Materials and Drugs used in Traditional Mongolian Medicines, Institute of Traditional Medicine and Technology of Mongolia, Jicom Press: Ulaanbaatar, p. 25-26,38,41,85, 93 (2023).
N. Tsagaanbaatar, U. Dashzeveg, N. Nyamdemberel, M. Urtnasan and C. Chimedtseren, Biomed. Pharmacol. J., 17, 1279 (2024); https://doi.org/10.13005/bpj/2941
H. Wagner and S. Bladt, Plant Drug Analysis: A Thin Layer Chromato-graphy Atlas, Springer-Verlag: Berlin, edn 2 (1996).
M. Ennajar, J. Bouajila, A. Lebrihi, F. Mathieu, M. Abderraba, A. Raies and M. Romdhane, J. Food Sci., 74, M364 (2009); https://doi.org/10.1111/j.1750-3841.2009.01277.x
E. Selenge, G. Odontuya and J. Batkhuu, Proc. Mong. Acad. Sci., 195, 48 (2010).
G.V. Radha, B. Sadhana, S.K. Trideva and S. Ganapaty, J. Pharmacogn. Phytochem., 8, 59 (2019).
J. Sukhdolgor, Plant Chemistry and Biochemistry, Ulaanbaatar (2013).
M.M. Lesjak, I.N. Beara, D.Z. Orèic, G.T. Anaèkov, K.J. Balog, M.M. Franciškovic and N.M. Mimica-Dukic, Food Chem., 124, 850 (2011); https://doi.org/10.1016/j.foodchem.2010.07.006