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Vol. 36 No. 12 (2024): Vol 36 Issue 12, 2024

Copyright (c) 2024 Thi Viet Huong Do, Minh Trang Vu, Minh Giang Phan, Anh Trung Trieu, Thi Minh Thu Nguyen, Thanh Hien Pham

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Isolation and Characterization of Bioactive Compounds from Hedyotis diffusa Willd. and Evaluation of their Antimicrobial, Antioxidant, Cytotoxic and Nitric Oxide Inhibitory Activities

https://doi.org/10.14233/ajchem.2024.32711
Vu Minh Trang
VNU University of Education, Vietnam National University, Hanoi, Vietnam
https://orcid.org/0000-0002-3504-6565
Trieu Anh Trung
Hanoi National University of Education, Hanoi, Vietnam
https://orcid.org/0000-0002-4220-9078
Tran Quang Tri
The Worthgate School, UK
Pham Thanh Hien
VNU University of Science, Vietnam National University, Hanoi, Vietnam
Nguyen Thi Minh Thu
VNU University of Science, Vietnam National University, Hanoi, Vietnam
https://orcid.org/0000-0002-6982-8071
Phan Minh Giang
VNU University of Science, Vietnam National University, Hanoi, Vietnam
https://orcid.org/0000-0003-4989-4183
Do Thi Viet Huong
a:1:{s:5:"en_US";s:84:"Faculty of Chemistry, VNU University of Science, Vietnam National University, Hanoi ";}
https://orcid.org/0000-0003-4709-4844

Corresponding Author(s) : Vu Minh Trang

trangvm@vnu.edu.vn

Asian Journal of Chemistry, Vol. 36 No. 12 (2024): Vol 36 Issue 12, 2024

Abstract

Hedyotis diffusa Willd. is traditionally used in Vietnamese and Chinese medicine for its therapeutic properties. This study aimed to explore the bioactive components of the ethyl acetate extract of Hedyotis diffusa Willd. and evaluate their potential antimicrobial, antioxidant and anticancer activities. The extract was collected using fractionated column chromatography and five compounds viz. cholestane, campesterol, stigmasterol, diosgenin and digitoxigenin were isolated and characterized by mass spectrometry and nuclear magnetic resonance spectroscopy. The extract exhibited significant antimicrobial activity against bacteria (Bacteroides fragilis, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pyogenes at 80 mg/mL) and fungi (Aspergillus flavus and Aspergillus parasiticus at 80 mg/mL) associated with liver diseases. The extract demonstrated inhibitory activity against nitric oxide (NO) production in LPS-stimulated RAW264.7 cells at 20 µg/mL with low cell death rates 37,12% and DPPH scavenging which may be attributed to its high phenolic content. These findings suggest that Hedyotis diffusa Willd. extract holds promise as a therapeutic agent for liver damage and cancer, potentially through mechanisms beyond antioxidant activity.

Keywords

Hedyotis diffusa Bioactive compounds Antimicrobial activity DPPH Cytotoxicity Nitric oxide inhibition
Trang, V. M., Trung, T. A., Tri, T. Q., Hien, P. T., Minh Thu, N. T., Giang, P. M., & Viet Huong, D. T. (2024). Isolation and Characterization of Bioactive Compounds from Hedyotis diffusa Willd. and Evaluation of their Antimicrobial, Antioxidant, Cytotoxic and Nitric Oxide Inhibitory Activities. Asian Journal of Chemistry, 36(12), 2837–2842. https://doi.org/10.14233/ajchem.2024.32711
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