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Vol. 25 No. 15 (2013): Vol 25 Issue 15

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Simultaneous Determination of Six Active Ingredients of Sanhuang Tablet by HPLC

https://doi.org/10.14233/ajchem.2013.14834
Weiming Cheng
Zhejiang Institute for Food and Drug Control, No. 86, Lane 1, Jichang Road, Jianggan Dist., Hangzhou 310004, Zhejiang Province, P.R. China
Jie Gao
Grade 2011, Zhejiang University of TCM, No. 548, Binwen Road, Binjiang Dist., Hangzhou 310053, Zhejiang Province, P.R. China
Hua Huang
Quzhou Institute for Food and Drug Control, No. 109, Hehuaer Road, Hecheng Dist., Quzhou 324002, Zhejiang Province, P.R. China
Bei Shi
Zhejiang Institute for Food and Drug Control, No. 86, Lane 1, Jichang Road, Jianggan Dist., Hangzhou 310004, Zhejiang Province, P.R. China
Qinwei Huang
Zhejiang Institute for Food and Drug Control, No. 86, Lane 1, Jichang Road, Jianggan Dist., Hangzhou 310004, Zhejiang Province, P.R. China
Shan Zhong
State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University (Guangzhou Medical University), No 151, YanJiang Road, Yuexiu Dist., Guangzhou 510120, Guangdong Province, P.R. China

Corresponding Author(s) : Shan Zhong

zhong-shan@126.com

Asian Journal of Chemistry, Vol. 25 No. 15 (2013): Vol 25 Issue 15

Abstract

An HPLC method for the simultaneous determination of six active ingredients present in famous Chinese clinical prescription has been established. HPLC conditions included Agilent Hypersil-C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was a mixture of acetonitrile and 0.1 % phosphoric acid for gradient elution. The flow rate was 1.0 mL/min and the column temperature was at 30 ºC. Six active ingredients had good linear relationships: berberine in the range of 0.0081-0.4045 μg (R2 = 0.9997), aloe-emodin in the range of 0.0076-0.3820 μg (R2 = 0.9999), rhein in the range of 0.0104-0.5175 μg (R2 = 0.9998), emodin in the range of 0.0122- 0.6110 μg (R2 = 0.9999), chrysophanol in the range of 0.0214-1.0690 μg (R2 = 0.9999) and physcion in the range of 0.0057-0.2830 μg (R2 = 0.9994). Their average recoveries were 104.73 % (RSD = 1.6 %), 95.19 % (RSD = 2.3 %), 104.31 % (RSD = 2.0 %), 101.72 % (RSD = 2.7 %), 95.50 % (RSD = 2.2 %) and 103.08 % (RSD = 2.7 %), respectively. This method was steady with high precision and good repeatability and could be used for the determination of the six active ingredients and quality control of Sanhuang tablet.

Keywords

HPLC Sanhuang tablet Berberine Aloe-emodin Rhein Emodin Chrysophanol Physcion
Cheng, W., Gao, J., Huang, H., Shi, B., Huang, Q., & Zhong, S. (2013). Simultaneous Determination of Six Active Ingredients of Sanhuang Tablet by HPLC. Asian Journal of Chemistry, 25(15), 8549–8551. https://doi.org/10.14233/ajchem.2013.14834
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