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Vol. 26 No. 11 (2014): Vol 26 Issue 11

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Study on CPS1: The Key Gene of Urea Cycle under the Stress of Aflatoxin B1

https://doi.org/10.14233/ajchem.2014.17515
Zhenhong Zhuang
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China;These authors contributed equally to this work
Yaning Lin
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China;These authors contributed equally to this work
Chi Yang
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China
Jun Yuan
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China
Shihua Wang
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China

Corresponding Author(s) : Shihua Wang

wshyyl@sina.com

Asian Journal of Chemistry, Vol. 26 No. 11 (2014): Vol 26 Issue 11

Abstract

Proteomic analysis of AFB1-stressed mouse liver revealed that the level of carbamoyl-phosphate synthetase 1 (CPS1) protein decreased. The study further explored the role of CPS1 in AFB1-induced liver pathological change in mice. Under AFB1 stress, the amount of blood ammonia was found increased, the expression of CPS1, OTC and SIR5 was found decreased with the increased concentration of AFB1 and CPS1 in Chang liver cell was found down-regulated by fluorescence quantitative PCR. When CPS1 in BEL-7402 cell and Chang liver cell was interfered, respectively, both of the level of OTC and the AFB1 tolerance of cell was found decreased. This study demonstrates the depression of CPS1, OTC and SIR5 genes under AFB1 stress. It inferred that the urea cycle could be interrupted by AFB1 and CPS1 played an important role in the process and that CPS1 and OTC can serve as candidate markers for liver pathological change under AFB1 stress.

Keywords

Aflatoxin B1 CPS1 Urea cycle
Zhuang, Z., Lin, Y., Yang, C., Yuan, J., & Wang, S. (2014). Study on CPS1: The Key Gene of Urea Cycle under the Stress of Aflatoxin B1. Asian Journal of Chemistry, 26(11), 3305–3310. https://doi.org/10.14233/ajchem.2014.17515
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