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Vol. 27 No. 1 (2015): Vol 27 Issue 1

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Direct Electrochemistry and Electrocatalysis of Hemoglobin Immobilized on Eggshell Membrane Modified Glassy Carbon Electrode

https://doi.org/10.14233/ajchem.2015.16820
Liangwei Du
College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P.R. China
Meiying Huang
College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P.R. China
Qiuhong Xu
College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P.R. China
Jiali Zhang
College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P.R. China

Corresponding Author(s) : Liangwei Du

dulily9@163.com

Asian Journal of Chemistry, Vol. 27 No. 1 (2015): Vol 27 Issue 1

Abstract

Eggshell membrane is a kind of naturally occurring organic material, which can be exploited as protein immobilization matrix. In this paper, eggshell membrane was effectively attached onto the surface of support substrates such as glass slide and glassy carbon electrode. Scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy were applied to characterize the supported eggshell membrane. Hemoglobin was immobilized in eggshell membrane and direct electron transfer was realized between protein and eggshell membrane modified electrode. Outer shell membrane could effectively facilitate direct electrochemistry of hemoglobin compared with the whole eggshell membrane. A pair of well-defined redox peaks of hemoglobin was obtained at outer shell membrane modified electrode. Hemoglobin immobilized in outer shell membrane showed a surface-controlled electrochemical process. Hemoglobin could still retain its catalytic activity for electrochemical reduction of O2 and H2O2. It is expected that eggshell membrane may find more potential applications in biosensors and biocatalysis.

Keywords

Eggshell membrane Hemoglobin Electron transfer Electrocatalysis
Du, L., Huang, M., Xu, Q., & Zhang, J. (2014). Direct Electrochemistry and Electrocatalysis of Hemoglobin Immobilized on Eggshell Membrane Modified Glassy Carbon Electrode. Asian Journal of Chemistry, 27(1), 153–157. https://doi.org/10.14233/ajchem.2015.16820
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