Separation of Racemates Using Chiral HPLC and Creation of a Database for this Methodology

Abstract

The selection of an appropriate system such as chiral stationary phase (CSP) and operating condition often remains difficult to find by trial and error. A database was initiated in HPLC to fill this gap. Two databases software were readily available as possible tools for achieving the objectives of the project. First, ISIS base was considered initially as a suitable tool for the project but the shortcoming was the disability to store chromatograms. Second, Microsoft Access was considered as an alternative, but it was unable to handle chemical information. So a secondary software called Accord for Microsoft Access was added to give chemical awareness. With the Accord add-in great chemical applications can create directly within Microsoft Access 97's. It gives a complete picture of the analyte including the chromatogram. The database was fed by information about the separation of twenty eight enantiomers and the separations have been carried out using the five chiral stationary phases (CSPs) available in the chemistry department at University of Manchester Institute of Science and Technology (UMIST). The database was examined for selection of a suitable column and the suitable conditions for chiral separation of enantiomers and it gave a successful and effective conclusion. Therefore this developed method save time, materials used for finding out the optimum conditions and labour.