Spectrophotometric Determination of Hemoglobin with Lanthanum(III)-(DBC-Arsenazo)

Abstract

A spectrophotometric determination of hemoglobin was   proposed with lanthanum(III)-(DBC-arsenazo) complex as a   spectrum probe in the presence of emulgent OP. In a Britton-   Robinson buffer at pH 2.9, hemoglobin, La(III) and DBCarsenazo   form a colour compound. At its maximum absorption   wavelength of 364 nm, the apparent molar absorptivity   for the determination of hemoglobin is ε₃₆₄ nm = 3.59 × 10⁵ L   mol^-1 cm^-1. The linear relationship between the concentration   of hemoglobin and its absorbance exists over the concentration   range of 10-70 mg L^-1 of hemoglobin and Beer's law is obeyed.   The proposed method was directly used in the determination   of total proteins in human serum samples with satisfactory   results.