Synthesis of Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Salbutamol from Urine Samples

Abstract

A highly selective methacrylic based molecularly imprinted polymer (MIP) was synthesized and applied for the separation and the pre-concentration of salbutamol in urine samples. Spectrophotometric determination of salbutamol was achieved using 2,6-dichloroquinone chlorimide as colorimetric reagent. The detection limit of the method was ca. 13 ng mL-1 in urine after pre-concentration of the samples by MIP-SPE and analysis with an optimized and sensitive spectrophotometric method. The linear dynamic range for salbutamol determination in urine was 0.04-0.75 μg mL^-1. The recovery for the affinity based solid-phase extraction (SPE) with the MIP was more than 96 %.