TY - JOUR AU - Lu, H.T. AU - Zou, Y.L. AU - Deng, R. AU - Shan, H. PY - 2013/11/11 Y2 - 2024/03/29 TI - Extraction, Purification and Antiradical Activities of Alpinetin and Cardamomin from Alpinia katsumadai Hayata JF - Asian Journal of Chemistry JA - ajc VL - 25 IS - 17 SE - Articles DO - 10.14233/ajchem.2013.15046 UR - https://asianpubs.org/index.php/ajchem/article/view/25_18_17 SP - 9503-9507 AB - <p style="text-align: justify;">Alpinetin and cardamomin are the major effective ingredients, which exist in <em>Alpinia katsumadai</em> Hayata and have strong activity in inhibiting platelet aggregation and tumor formation, antiinflammatory and antibacterial activities. Using orthogonal experiments L<sub>16</sub> (4<sup>3</sup>), alpinetin and cardamomin were extracted from <em>Alpinia katsumadai</em> Hayata by microwave-assisted extraction. Then, high-speed counter-current chromatography (HSCCC) was used to separate and isolate of alpinetin and cardamomin by two-phase solvent system of <em>n</em>-hexane-ethyl acetate-ethanol-water (5:6:5:5, v/v) in one-step separation. The lower phase was used as the mobile phase in the head-to-tail elution mode. From 158.4 mg of the crude extract, 14.47 mg of alpinetin and 1.72 mg of cardamomin with purities of 99.01 and 97.71 % were obtained, respectively. They were identified by melting points, UV, IR, <sup>1</sup>H and <sup>13</sup>C NMR. Furthermore, they were tested for 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals scavenging activities. The results showed that alpinetin had strong DPPH free radicals scavenging activities.</p> ER -